Dozens of vehicles are blocking key roads across Myanmar's main city Yangon, in what appears to be a new tactic employed by protesters.
It comes as tens of thousands of people gather downtown, in what organisers hope will be the biggest protest yet.
The UN special rapporteur for Myanmar, also known as Burma, has warned of potential violence.
Demonstrators are demanding the release of their elected leaders following a 1 Feb military coup.
Aung San Suu Kyi and other leaders have been detained by the military.
Today's protests, which have nearly paralysed traffic in downtown Yangon, come just a day after Ms Suu Kyi was handed a second criminal charge.
Myanmar's military had also on Tuesday repeated its promise to hold fresh elections and relinquish power, though many protesters remain sceptical.
A campaign on social media calling for protesters to deliberately block roads started gaining traction early on Wednesday. Its aim was apparently to stop civil servants from going to work and hinder the movements of security forces.
Dubbed "Road Blocking Day", it has seen many posting pictures online of vehicles with raised bonnets and boots parked across several roads in Yangon, making the streets impassable to traffic.
A BBC Burmese reporter saw a number of public buses stop at junctions in Yangon and taking part in what some have called the "broken down car" movement.
The protest is the latest in a mounting civil disobedience movement in the country, which has also seen strikes by doctors and teachers, and boycotts of products and services owned by the military.
IMAGE COPYRIGHTGETTY IMAGES
image captionProtesters block a bridge with their cars during a demonstration against the military coup
But one activist criticised the road blockages, saying it was holding up protesters heading downtown.
"Stop the 'broken down car' and help the protesters get to Sule as soon as possible," said activist Maung Saung Kha according to a Reuters report.
Tens of thousands of people have flooded the Sule area to take part in a major protest.
Demonstrators have gathered almost every day for the past fortnight, but organisers are hoping that Wednesday's protest will see the biggest turnout yet.
IMAGE COPYRIGHTEPA
"Let's gather in millions to take down dictators," Khin Sandar, a senior member of Aung San Suu Kyi's National League for Democracy had posted on Facebook.
"Let's march en masse [and] show our force against the coup government that has destroyed... the future of our country."
The UN Special Rapporteur on the situation of human rights in Myanmar Tom Andrews warned of "potential for violence on a greater scale" on Tuesday, adding that additional soldiers had been deployed in cities where protests were planned.
"I am terrified that given the confluence of these two developments - planned mass protests and troops converging - we could be on the precipice of the military committing even greater crimes against the people of Myanmar."
IMAGE COPYRIGHTEPA
Myanmar's military seized control of the country on 1 February, following a general election which saw Ms Suu Kyi's NLD party win by a landslide.
It claimed that the NLD had won through fraud, and demanded a rerun of the vote - despite the electoral commission saying there was no evidence to support these claims.
Power has now been handed over to commander-in-chief Min Aung Hlaing, and Ms Suu Kyi is under house arrest.
She was earlier charged with possessing illegal walkie-talkies, and has now been accused of violating the country's Natural Disaster Law, though the details of this second charge are unclear.
Protesters are demanding the release of Ms Suu Kyi and other key NLD members - and protests over the coup have been the largest since the so-called Saffron Revolution in 2007.
media captionMyanmar coup: How did we get here?
But clashes have taken place between security officers and protesters, and there are reports of police using tear gas and rubber bullets to disperse crowds.
The military has also been regularly blocking the internet in a bid to stifle dissent.
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